报告题目：The bacterium Wolbachia changes host reproduction and inhibits viral infection in mosquito
报 告 人：Zhiyong Xi博士，Michigan State University（美国）
Diseases transmitted by blood-feeding arthropod vectors, such as mosquito-borne malaria and dengue fever, cause 1.5 million human deaths every year. The insufficiency of currently available strategies, including vaccines, drugs, and pesticides, has led to an increase in vector-borne diseases. The endosymbiotic bacterium Wolbachia is widely recognized for its potential as a vehicle to introduce disease-resistance genes into mosquitoes, making them refractory to the human pathogens they transmit. This is due to its ability to induce a reproductive abnormality known as cytoplasmic incompatibility (CI). CI is early embryo death when a Wolbachia infected male mates with a female that is uninfected or harboring a different Wolbachia type. Since uninfected males can successfully mate with infected females, Wolbachia and any gene it carries can spread quickly in a population. We recently observed that Wolbachia induces resistances to dengue virus in mosquito Aedes aegypti. This provides Wolbachia a “mosquito vaccine” like feature, which can be introduced, driven through CI, and spread over mosquito population to block transmission of mosquito-borne diseases. I will present our recent works on how Wolbachia-mosquito interactions activate Toll pathway and induce expression of anti-dengue effectors. The mosquito gene expression profile regulated by Wolbachia will be dissected in the presence and absence of dengue virus, with special focus on immune and redox genes. Finally, a three-way interactions between Wolbachia, dengue virus and mosquito host will be analyzed in mosquito protein interaction network to further understand the molecular mechanisms of Wolbachia-induced CI and viral interference. I will discuss the significance of our findings for pharmacological intervention and development of environmentally friendly biopesticides and novel strategies for future control of vector-born disease.